Intravital Imaging of the Immune-system
Oct 14, 2020 7:30 AM
Bernd H. Zinselmeyer, PhD
Intravital Imaging of the Immune-system

My scientific education has provided me with a broad and interdisciplinary research background spanning biochemistry, molecular biology, and cellular biology techniques. For example, I have acquired applied skills in chemical and physiochemical techniques such as structural NMR and photon correlation spectroscopy and have used these skills to design, synthesize, and characterize novel compounds. I also have solid expertise in DNA/RNA cloning strategies as well as working with small animal models of infection and inflammation. Earning my Ph.D. in pharmaceutical science has inspired me to focus on translational science, with an emphasis on research that has relevance to clinical medicine. The main focus of my work over the past 8 years has been intravital multiphoton imaging of cells of the immune and the central nervous system.

I have built, optimized, and modified confocal microscopes and attached two-photon lasers to perform intravital imaging. Besides the demanding technical aspect, an additional challenge in
the intravital imaging field is the appropriate processing of the tremendous amount of data collected within each experiment/study. One of the key approaches here is the development of methods and tools for the analysis of 4-dimensional data. Since my time in Scotland, I have successfully continued collaborating with Dr. John Dempster at Strathclyde University to develop software capable of solid analysis of these novel data.